Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | endogenous HlLgm2 expression in the midgut that is upregulated by the blood-feeding process is shown in all the developmental stages | Haemaphysalis longicornis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Haemaphysalis longicornis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | papain-like cysteine proteinase inhibitor, 28.77% inhibition at 0.1 mM concentration relative to control | Haemaphysalis longicornis | |
Ca2+ | moderate inhibition | Haemaphysalis longicornis | |
Co2+ | strong inhibition | Haemaphysalis longicornis | |
Cu2+ | strong inhibition | Haemaphysalis longicornis | |
cystatin | from egg white, 62.3% inhibition at 0.003 mM concentration relative to control | Haemaphysalis longicornis | |
E-64 | papain-like cysteine proteinase inhibitor, 7.51% inhibition at 0.3 mM concentration relative to control | Haemaphysalis longicornis | |
Fe2+ | strong inhibition | Haemaphysalis longicornis | |
iodoacetamide | 100% inhibition at 3 mM concentration relative to control | Haemaphysalis longicornis | |
leupeptin | papain-like cysteine proteinase inhibitor, 3.9% inhibition at 0.3 mM concentration relative to control | Haemaphysalis longicornis | |
Mg2+ | moderate inhibition | Haemaphysalis longicornis | |
Mn2+ | moderate inhibition | Haemaphysalis longicornis | |
N-ethylmaleimide | 100% inhibition at 3 mM concentration relative to control | Haemaphysalis longicornis | |
Ni2+ | strong inhibition | Haemaphysalis longicornis | |
pepstatin A | aspartic peptidase inhibitor has minimal effects on the enzyme activity, 12.22% inhibition at 0.3 mM concentration relative to control | Haemaphysalis longicornis | |
PMSF | serine peptidase inhibitor, 13.84% inhibition at 5 mM concentration relative to control | Haemaphysalis longicornis | |
Zn2+ | moderate inhibition | Haemaphysalis longicornis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
endogenous HlLgm2, predicted and confirmed by SDS-PAGE and immunoblot analysis | Haemaphysalis longicornis |
42500 | - |
His-tagged rHlLgm2, determined by SDS-PAGE | Haemaphysalis longicornis |
47490 | - |
predicted preprotein | Haemaphysalis longicornis |
49630 | - |
predicted preproprotein | Haemaphysalis longicornis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bovine albumin + H2O | Haemaphysalis longicornis | - |
peptides containing C-terminal Asn | - |
? | |
bovine haemoglobin + H2O | Haemaphysalis longicornis | exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide | peptides containing C-terminal Asn | - |
? | |
additional information | Haemaphysalis longicornis | enzyme is involved in host blood-meal digestion | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemaphysalis longicornis | A9CQC1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | asparagine-linked glycosylation | Haemaphysalis longicornis |
proteolytic modification | the values expected on the basis of the predicted MW indicating that post-translational processing of HlLgm2 occurred during biosynthesis to form a mature protein through removal of 9 kDa peptides from the precursor protein, putative C-terminal autocleavage at site Asn364-365 | Haemaphysalis longicornis |
Purification (Comment) | Organism |
---|---|
recombinant protein is purified using Ni-Sepharos under native conditions | Haemaphysalis longicornis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Haemaphysalis longicornis | - |
larva | - |
Haemaphysalis longicornis | - |
midgut | - |
Haemaphysalis longicornis | - |
nymph | - |
Haemaphysalis longicornis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000133 | - |
hydrolysis of substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methyl-coumaryl-7-amide | Haemaphysalis longicornis |
0.00041 | - |
hydrolysis of substrate acetyl-aspartyl-asparaginyl-leucyl-aspartic acid alpha-(4-methylcoumaryl-7-amide) | Haemaphysalis longicornis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Asp-Asn-Leu-Asp alpha-(4-methylcoumaryl-7-amide) + H2O | - |
Haemaphysalis longicornis | Ac-Asp-Asn-Leu-Asp + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Haemaphysalis longicornis | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
bovine albumin + H2O | - |
Haemaphysalis longicornis | peptides containing C-terminal Asn | - |
? | |
bovine haemoglobin + H2O | exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide | Haemaphysalis longicornis | peptides containing C-terminal Asn | - |
? | |
additional information | enzyme is involved in host blood-meal digestion | Haemaphysalis longicornis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asparaginyl endopeptidase | - |
Haemaphysalis longicornis |
HlLgm2 | - |
Haemaphysalis longicornis |
legumain | - |
Haemaphysalis longicornis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Haemaphysalis longicornis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the rHlLgm2 is more active in neutral to slightly alkaline condition | Haemaphysalis longicornis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haemaphysalis longicornis | predicted preprotein | - |
6.7 |
Haemaphysalis longicornis | predicted preproprotein | - |
7.1 |